CTGF-treated MSCs were α-SMA–, differentiating into α-SMA+ myofibroblasts only when stimulated subsequently with TGF-β1, suggestive of stepwise processes of fibroblast commitment, fibrogenesis, and pathological fibrosis. The system enabled hn-MSC differentiation into HLCs which was certified by positive immune staining of hepatic markers, the hepatic transporters, and drug-metabolizing enzymes. Total RNA was isolated from confluent cultures of three lines of MSC (passage 6–9), three lines of fibroblasts (passage 7–14), three lines of MSC incubated for 24 h or 28 days in the differentiation‐induction medium, or three lines of osteoarthritic synovium‐derived MSC (passage 6–8), using RNeasy Mini Kit (Qiagen, Chatsworth, CA). Total protein content was determined by BCA method 34. Interestingly, the differences observed between the two cells have also been observed between young and old MSCs. In comparison to CB- and BM-derived cells, UC cells revealed an absent trilineage differentiation capacity in vitro. … Culture of BM-MSC or fibroblasts under conditions favouring adipogenic differentiation for 21 days was followed by staining with Oil Red O (Scale, 100μm) (B) . Characterization of human adMSC. Furthermore, MSCs can undergo osteogenic, adipogenic and chondrogenic differentiation … Table 1 … In addition, it appears to differentiate MSCs into a more fibroblast-like cell type. The possibility that fibroblasts can also differentiate into tissue is intriguing. These data suggest that the tested 3D models could improve HLC maturation, showing a relevant … To address inherent heterogeneity in MSC culture, we established 18 single MSC-derived clones by limiting dilution. Adult bone marrow-derived mesenchymal stem cells (MSCs) are able to differentiate into myofibroblasts and be recruited into wound lesions and contribute to wound healing. Mesenchymal stem cell (MSC)-based therapies have been used in skin regeneration due to their ability to differentiate into many cells, promote cytokine secretion and participate in collagen deposition. To address inherent heterogeneity in MSC culture, we established 18 single MSC–derived clones by limiting dilution. Multipotent mesenchymal stromal cells (MSCs) secretome can interfere with fibrosis development; despite precise underlying mechanisms remain unclear. inflammatory sites where they differentiate into connective tissue cells, such as adipocytes, chondrocytes and osteocytes [14]. Of note, no protocols to convert fibroblasts or into MSC by MSC-specific transcription factors have been reported up to now, ... Fibroblast-derived MSC-differentiation into pericytes was further confirmed by co-immunostaining of the pericytes/smooth muscle cell marker smooth muscle actin (ACTA2) and the reporter protein Turquoise 2/CFP (Fig. Local resting resident fibroblasts are activated after injury and play a critical role in … The lack of potential to differentiate into chondrocytes was documented by Alcian-Blue periodic acid-Schiff, Safranin O staining, and real-time PCR analysis of SOX9. A cell can be classified as an MSC if it shows plastic adherent properties under normal culture conditions and has a fibroblast-like morphology. @article{osti_22207513, title = {Differentiation of human umbilical cord mesenchymal stem cells into dermal fibroblasts in vitro}, author = {Han, Yanfu and Chai, Jiake and Sun, Tianjun and Li, Dongjie and Tao, Ran}, abstractNote = {Highlights: {yields} Mesenchymal stem cells (MSCs) are potential seed cells for tissue-engineered skin. For example, Sabatini et al. found that fibroblast-like cells cultured from human adult bronchus had surface markers similar to bone marrow-derived MSC (with the exception of Sto-1, which was expressed weakly on bronchial fibroblasts) and comparable differentiation into bone, fat and cartilage, with collagen and extracellular matrix production. Under appropriate culture conditions, adMSC can differentiate into adipocytes and osteoblasts as demonstrated by Oil red O staining and ALP staining, respectively (Fig. In this study, we demonstrate that mouse embryonic stem cells (mESCs) can differentiate into cells with MSC properties. The increased differentiation potential … Using previously established methods that characterize Ad-MSCs, we analyzed mESC-differentiated fibroblasts (mESC-FBs), including plastic adherence, clonogenic growth, MSC marker expression, tri-lineage differentiation potential, and the capacity to express … DNA microarray analysis was carried out by … Although fibroblasts in heterogeneous MSC culture may have been stimulated to proliferate by CTGF, single MSC progenies were indeed capable of differentiating into fibroblastic cells. We investigated which PDAC cell line effectively recapitulated clinical morphology, including aggressive tumor growth and CAF remodeling in vivo. In addition, all models displayed relevant glucose metabolism, ALB production, and urea secretion. PCa cells, MSC, fibroblasts or macrophages derived from experimental conditions were lysed for 20 minutes on ice in 500 μl of RIPA lysis buffer (50 mM Tris–HCl, pH 7.5, 150 mM NaCl, 1% Triton X‐100, 2 mM EGTA, 1 mM sodium orthovanadate, 1 mM phenylmethanesulphonyl‐fluoride, 10 μg/ml aprotinin, 10 μg/ml leupeptin). … Background information: Although MSCs (mesenchymal stem cells) and fibroblasts have been well studied, differences between these two cell types are not fully understood. Clonal progenies of MSCs differentiate into mesenchymal lineages. The only difference between surface markers is … This notion is supported by an experimental paradigm described more than a decade ago, when it was shown that normal morphogenesis and differentiation of human breast epithelial cells transplanted into mice required co-implantation with human fibroblasts . The therapeutic significance of the ability of MSC to differentiate into various tissues is under debate, with some arguments being that MSC exert disease inhibiting activity by secretion of soluble factors, while other schools of thought suggest that MSC actually differentiate into injured tissue. 2. Cell-based therapies (CBTs) are quickly taking hold as a revolutionary new approach to treat many human diseases. In fact, some argue that MSCs and fibroblasts are functionally identical. No significant difference was found even in surface markers, one of the most useful description elements. Fibroblasts differentiation into myofibroblasts is a central event of tissue fibrosis. In hepatocyte-like cells obtained after hAM-MSC differentiation, a decreased expression of the alpha-fetoprotein, a marker of fetal ... hepatocytes appears to be safer than use of native MSC due to the fact that the use of native MSC may result in their differentiation into fibroblasts, which gather in fibrous septa, thus acting profibrotically [172, 178]. It was also observed that FGF2 stimulated the activation of the phosphorylation of ERK1/2 leading to the upregulation of essential extracellular matrix molecules and cytoskeletal elements for ligaments and tendons. Patent US8822215B2 - Differentiation of mesenchymal stem cells into fibroblasts, compositions comprising mesenchymal stem cell-derived fibroblasts, and … Nonetheless, without any other marker to differentiate between the cells in the first place, it would be difficult to find a definitive marker. In this study, we concluded that a CuS@BSA nanoparticles exhibited similar potential in inducing MSCs differentiation to fibroblasts as Cu ions for wound healing. These cloning data further substantiate the innate capacity of MSCs to differentiate into common connective tissue phenotypes, including bone, cartilage, and adipose. Also, in recent years it has been showed that fibroblasts also have differentiation capacity as MSCs and can differentiate into several cell types such as osteoblasts, chondrocytes, adipocytes, hepatocytes, neuron-like cells, etc. Knowledge gained from this differential genes expression comparison will lead to a better … Three human FB populations (HLF, WI38, and NHDF) were switched from growth medium to osteoblast or adipose differentiation media and assayed at 7 or 14 days. cell differentiation into, many types like osteoblasts, adipocytes, and chondrocytes. First, we examined the histological features of the tumor in an in vivo xenograft mouse. In this study, to study the effects of MSC on the collagen metabolism of fibroblasts, we isolated MSC from human adipose tissues. The cellular and molecular mechanisms responsible for MSC trafficking and differentiation, however, are poorly understood. In comparison to CB- and BM-derived cells, UC cells revealed an absent trilineage differentiation capacity in vitro. 2c, d). To address the notion that MSC-derived fibroblastic cells arise from fibroblasts in typical heterogeneous MSC culture, we iso-lated clones from MSCs and determined their lineage commit-ment and differentiation potential. Hence, the future goal of tissue engineering (TE) is the replacement and repair of lost muscle tissue by newly generating skeletal muscle combining different cell sources, such as myoblasts and mesenchymal stem cells (MSCs), within a three-dimensional matrix. Human primary FBs, like MSCs, can differentiate in vitro into bone or adipose under the appropriate conditions, although the differentiation potential is variable. Two BM‐MSCs were treated similarly in the same experiment. Fig. In-vitro aging MSCs gradually lost the typical fibroblast-like spindle shape, leading to elevated morphological abnormality and inhomogeneity. The lack of potential to differentiate into chondrocytes was documented by Alcian-Blue periodic acid-Schiff, Safranin O staining, and real-time PCR analysis of SOX9. Furthermore, neither endothelial nor myogenic differentiation was documented after induction of UC-MSC. a Immunophenotype of human … Accordingly, hESC-MSCs might have the potential of fibroblast differentiation, and they could be unlimited cell sources of fibroblasts to overcome the drawbacks of currently existing treatments for pressure ulcers, considering that human MSCs can be differentiated into fibroblasts using connective tissue growth factor (CTGF; also known as CCN2) [31, 32]. CTGF-treated MSCs were alpha-SMA-, differentiating into alpha-SMA+ myofibroblasts only when stimulated subsequently with TGF-beta1, suggestive of stepwise processes of fibroblast commitment, fibrogenesis, and pathological fibrosis. And fibroblasts also can differentiate into variety of fibroblast types. MSC are being use in the clinic for the treatment of a variety of clinical conditions. Volumetric muscle loss caused by trauma or after tumour surgery exceeds the natural regeneration capacity of skeletal muscle. Capan‐1‐induced MSC differentiation into CAFs. Moreover, further studies propose that these cells have potential to transdifferentiate into several cell types, including endodermal and ectodermal lineages [15]. Giving the more readiness of MSCs-derived NPCs to differentiate into neurons and glial cells compared with MSCs, unravelling differential genes expression between MSCs and MSC-derived NPCs will lead to a better understanding of the molecular processes that govern this differentiation. The gene expression profile and adipogenic and osteogenic differentiation capability of MSCs at early (passage 4) and late (passage 8) passages were also evaluated. Furthermore, neither endothelial nor myogenic differentiation was documented after induction of UC-MSC. This also seems to be true of certain cell surface markers. 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