A fresh, well-made, peripheral blood film is crucial for accurate cell morphology assessment. The examination of the blood is useful in the diagnosis of many conditions, including leukemia, lymphoma, and myeloproliferative disorders. This is inexpensive, easy to perform and rapidly done as a screening test. A reproducible blood smear review requires every peripheral smear be prepared for consistent cellular distribution and proper clarity. Slides are just automatically dipped in the stain in the buffer and a series of rinses. Staining Procedure: Quality Control To ensure that proper staining results have been achieved, a positive smear (malaria) should be included with each new batch of working Giemsa stain. A variety of methods are available for preparing and staining blood smears, the most common of which are discussed in this atlas. It takes about 5-10 minutes to stain a batch of smears. Blood films are examined in the investigation of hematological (blood) disorders and are routinely employed to look for blood parasites, such as those of malaria and filariasis Fig: 3 staining technique of Peripheral blood smear The smear is floored with stain for about 5-10 minutes, then double diluted with buffered water and allowed for another 5–10 minutes for the cells to pick the stain. Peripheral blood smear examination is an invaluable investigation for the diagnosis of various clinical diseases, it includes cellular ... temperature and humidity also affects the staining of smear.Thus before reporting of smear pathologist must be vigilant of all these requirements. The risk is increased in smears made with anticoagulated blood. Focus a well stained area where the cells are uniformly distributed without rouleaux formation.Put two drops of cedar wood oil on the smear and examine under oil immersion objective (with plane mirror, condenser raised and iris diaphragm fully opened).lt is ideal to prepare and stain 2 or 3 peripheral blood smears simultaneously. Methodology Put the smear into mehthanol jar and fix it for 1 minute. E.D.T.A. Peripheral Blood Smear. The blood film is one of the world's most widely and frequently used tests and has undergone remarkably few changes since its introduction in the late 1800s. Finger Prick or . Peripheral smear 2. Staining of peripheral blood smears with methyl-green-pyronin, as used in this study, yielded some interesting and important information not apparent with the routine Wright’s stain. BLOOD SMEAR BASICS JENNIFER A. NEEL, DVM, DACVP (CLINICAL) ASSOCIATE PROFESSOR, CLINICAL PATHOLOGY NC STATE COLLEGE OF VETERINARY MEDICINE RALEIGH, NC, 27607 Introduction Although tremendous advances have been made in the field of point-of-care hematology analyzers, examination of a well prepared, well stained blood smear remains the cornerstone of veterinary PROCEDURE OF GIEMSA STAINING. This article gives direction and some standardization in the preparation of blood films used for morphologic evaluation in the clinical labora … A poor slide is a torment. This procedure is applicable for either hand or automated staining processes. Download Blood Smear Staining Protocol doc. Blood films that are too thin or too thick present a problem. The thin blood smear was further fixed in absolute methanol for 3seconds and allowed to air dried. The blood smear is a simple blood test. 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